The differentiation of the stem cells can be guided to produce desired cell type and regenerate damaged tissues, upon their transplantation. These potentials of stem cell therapy in
Development of a novel technique now offers stem cell generation from the blood samples of dogs, that seeds the hope to advance regenerative therapies in veterinary medicine, by a team of scientists from Osaka Prefecture University in Japan, published in the journal Stem Cells and Development.
Regerantive Therapy in Veterinary Medicine
The team had been working on isolating “induced pluripotent stem cells” (iPSCs) from canine blood samples. iPSCs are a type of stem cell that can be “programmed” from a developed (or “differentiated”) cell by introducing a specific set of genes into them.
These gene-encoded specific proteins called “transcription factors,” that can mature into various cell types. “We successfully established an efficient and easy generation method of canine iPSCs from peripheral blood mononuclear cells. It may be possible to perform regenerative medicinal treatments in dogs”, says Dr. Shingo Hatoya, Associate Professor from Osaka Prefecture University.
The team developed “footprint-free” stem cells by using a particular type of viral vector that can generate iPSCs without genomic insertion that cause tumor formation. This is automatically “silenced” via “microRNAs” expressed by the cells.
These cells were then grown in a special type of medium that contained various factors enhancing their pluripotency (including a “small-molecule cocktail” ). The team thereby successfully paved the way for an easy stem cell therapy technique by developing the germ layers, which form the basis of all organs.
“We believe that our method can facilitate the research involving disease modeling and regenerative therapies in the veterinary field. Dogs share the same environment as humans and spontaneously develop the same diseases, particularly genetic diseases”, says Dr. Hatoya.
The study affirms that further research into regenerative therapies for canines might help find treatments and even cures, for some of the diseases that still plague humanity.